🧫 Plasmid Map Designer — ORF + restriction sites
Анализ плазмидных последовательностей: поиск ORF (ATG/GTG/TTG → stop на обеих цепях), сайты рестриктаз, GC content. Визуализация circular map.
Образцы
Ферменты
Последовательность
Длина
569 bp
GC%
59.4%
ORF
9
Cut sites
49
Circular map
Open Reading Frames (top 10)
| # | Start | End | Len (bp) | Strand | Protein (first 30 aa) | GC% |
|---|---|---|---|---|---|---|
| 1 | 155 | 565 | 411 | - | LHACRSTLEDPRVPSSNSDPSLHACRSTLE... | 58.9% |
| 2 | 155 | 502 | 348 | - | LHACRSTLEDPRVPSSNSDPSLHACRSTLE... | 58.9% |
| 3 | 155 | 439 | 285 | - | LHACRSTLEDPRVPSSNSDPSLHACRSTLE... | 58.9% |
| 4 | 155 | 376 | 222 | - | LHACRSTLEDPRVPSSNSDPSLHACRSTLE... | 59% |
| 5 | 155 | 313 | 159 | - | LHACRSTLEDPRVPSSNSDPSLHACRSTLE... | 59.1% |
| 6 | 56 | 196 | 141 | + | LMTSTSSVPGDPLESTCRHASLDPNSSSVP... | 60.3% |
| 7 | 59 | 196 | 138 | + | MTSTSSVPGDPLESTCRHASLDPNSSSVPG... | 60.9% |
| 8 | 155 | 250 | 96 | - | LHACRSTLEDPRVPSSNSLVRPQACMPAGR... | 59.4% |
| 9 | 119 | 196 | 78 | + | LDPNSSSVPGDPLESTCRHASLRPH* | 60.3% |
Restriction sites (49)
SmaI @17BamHI @22PstI @40HindIII @52SmaI @80BamHI @85PstI @103HindIII @115BamHI @121EcoRI @127SmaI @143BamHI @148PstI @166HindIII @178EcoRI @198SmaI @214BamHI @219PstI @237HindIII @249BamHI @255EcoRI @261SmaI @277BamHI @282PstI @300HindIII @312BamHI @318EcoRI @324SmaI @340BamHI @345PstI @363HindIII @375BamHI @381EcoRI @387SmaI @403BamHI @408PstI @426HindIII @438BamHI @444EcoRI @450SmaI @466BamHI @471PstI @489HindIII @501BamHI @507EcoRI @513SmaI @529BamHI @534PstI @552HindIII @564